Improving Cell Infiltration in Electrospun Nanofibers

It is common knowledge that in order to biomimic the extracellular matrix (ECM) structure, electrospun fibers can be created with fiber diameter ranging in the nano- to micro-scale. Meanwhile, cell infiltration into scaffolding material is usually limited by pore size in its structure. In order to address this mishap, the scientific community has worked with various methods to increase pore size and porosity. Some of these include co-electrospinning fibers in the nano- and micro-scale at the same time, incorporating salt particles during fiber formation to eventually leach them out, co-electrospinning a water and non-water soluble fibers, and even the introduction of channels into the spun structure with laser ablation. Although these can successfully render large pores into the fibrous scaffold, limitations on reproducibility and homogeneity can still be a disadvantage.

当细胞在电纺支架中培养时，由于氧气和养分运输有限，它们的生存能力通常为100 µm。为了快速提高电纺支架上的细胞密度，基于同时的电纺纤维和电抛光细胞的组织构建体的制造已在组织工程和再生医学中更常用。下图显示了一张图表，说明了如何与电喷雾细胞同时创建电纺纤维。

Electrospraying Cells into Electrospun Fibers: Cell Viability After Voltage Induction

It has been widely demonstrated that the survival rate of various cells that underwent high voltages during the electrospray process is higher than 95 %. By simultaneously electrospraying cells into electrospun fibers, cell viability and incorporation at different sample depth can be improved by co-depositing at the same time. A bar plot demonstrating the viability of cardiosphere-derived cells (CDCs) electrosprayed at different voltages (10, 15 and 20 kV) and cultured up to 5 days proves similar proliferation results to the non-exposed at high voltages.

The following image shows the distribution of CDCs that were electrosprayed simultaneously with electrospun material that is able to mimic the cardiac ECM structure. It is shown that at different thicknesses cell distribution is homogeneous and viable after 1 (a) and 7 (b) days of culture. TheFluidnatek® equipment不仅允许同时形成电纺纤维和电喷雾细胞，还可以通过其工业制造设备来扩展此过程。通过实施HEPA或ULPA过滤器以及UV-C灯来维持无菌条件的环境控制单元，多轴功能，超级工作条件以及UV-C灯的结合，这些单元能够在组织工程时最大化并成为最终的多功能包装需要采用再生医学应用。188金宝搏app安卓下载